GLUTAMINE SYNTHETASE EXPRESSION AND IMMUNOLOCALIZATION IN THE DEVELOPING RAT LUNG. † 1831

Transferrin gene expression and transferrin immunolocalization in developing foetal rat lung.

In previous studies we have shown that transferrin (Tf) specifically stimulates dermatan- and chondroitin-sulphate proteoglycan accumulation around lung cells, and in the extracellular matrix of lung tissue, in vitro. The aim of this study was to determine whether the gene for Tf was activated in specific lung cells during development, and whether the protein product showed evidence of associat...

Dexamethasone regulates glutamine synthetase expression in rat skeletal muscles.

The regulation of glutamine synthetase expression by dexamethasone was studied in rat skeletal muscles. Daily administration of dexamethasone caused striking enhancement of glutamine synthetase activity in plantaris, soleus, and diaphragm muscles. Northern blot analysis revealed that the dexamethasone-mediated increase of glutamine synthetase activity was associated with dramatically increased ...

Glutamine Synthetase. 3. Factors Controlling Its Activity in the Developing Rat.

The developmental changes in glutamine synthetase activity in rat liver and brain have been studied and shown to be different in the two tissues. The liver enzyme exhibited a fall in activity after birth, followed by a slow recovery and a rapid increase in activity for a short interval of time, before a gradual rise to the adult level in about 40 days. The brain enzyme raised its activity gradu...

Mechanisms governing the expression of the enzymes of glutamine metabolism--glutaminase and glutamine synthetase.

Whether on the scale of a single cell, organ or organism, glutamine homeostasis is to a large extent determined by the activities of glutaminase (GA, EC 3.5.1.2) and glutamine synthetase (GS, EC 6.3.1.2), the two enzymes that are the focus of this report. GA and GS each provide examples of regulation of gene expression at many different levels. In the case of GA, two different genes (hepatic- a...

Biochemical and molecular analysis of glutamine synthetase gene in brassica napus L.